| Insect viruses produce large, proteinaceous particles called polyhedra, which function to protect progeny virions from hostile environmental conditions.
Considering this biological function of polyhedra, we hypothesized that occlusion into polyhedra might be a useful way to immobilize recombinant proteins while retaining their functions. Occlusion also might protect recombinant proteins and permit the production of stable protein libraries. Finally, the polyhedra containing recombinant proteins, Protein beads, might be fixed onto an inert surface in such a way as to permit their specific detection using conventional methods, such as immunohistochemistry.
The PCC's technology is a unique protein expression system consisted of automatic immobilization of the expressed protein molecules into insect viral occlusion bodies (polyhedra). Thus, the polyhedra which immobilize foreign protein molecules are called Protein beads. The Protein beads protect the immobilized protein from desiccation or higher temperature. Therefore, the Protein beads are very useful for establishment of a protein bank or a protein library, and it can be expected that the PCC's technology can contribute to the field of life science, especially proteome analysis.
US patent number 7,432,347 (Oct. 7, 2008)
US patent number 7,619,060 (Nov. 17, 2009)