Protein Crystal Corporation

Cytokine-polyhedra and cells

Recent news!

1) Control of angiogenesis by protein beads!
Matsumoto, G., Hirohata, R., Hayashi, K., Sugimoto, Y., Kotani, E., Shimabukuro, J., Hirano, T., Nakajima, Y., Kawamata, S., Mori, H. (2014) Control of angiogenesis by VEGF and endostatin-encapsulated protein microcrystals and inhibition of tumor angiogenesis. Biomaterials 35, 1326-1333 (2014)
This link will provide free access to this article, and is valid for 50 days, until 26th March, 2014.
2) Bone regeneration by BMP-2 protein beads
Our new paper of BMP-2 protein beads “Bone regeneration by polyhedral microcrystals from silkworm virus” has been published in Scientific Reports.
This paper is open access and you can freely download it.

3) Culture of human naive iPS cells by using of LIF protein beads.
Nishishita, N., Ijiri, H., Takenaka, C., Kobayashi, K., Goto, K., Kotani, E., Itoh, T., Mori, H., Kawamata, S. (2011) The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells.

Biomaterials 32, 3555-3563.

4) Medical applications of protein beads.
Matsushima, K., Suyama, T., Takenaka, C., Nishishita, N., Ikeda, K., Ikada, Y., Sawa, Y., Jakt, L.-M., Mori, H., Kawamata, S. (2010) Secreted frizzled related protein 4 reduces fibrosis scar size and ameliorates cardiac function after ischemic injury.

Tissue Engineering Part A. 16, 3329-3341.

5) A new method for protein beads.
A new method for the immobilization of foreign proteins into CPV polyhedra was developed by collaboration with Auckland Univ. and Kyoto Institute of Technology. Fibroblast growth factor2 (FGF2), FGF7 (keratinocyte growth factor (KGF)), and epidermal growth factor (EGF) were immobilized into polyhedra. Biological activities of the immobilized FGF2, FGF7, and EGF were assayed and compared with the conventional method (US patent No. 7432347). FGF7 and EGF polyhedra (FGF7 and EGF protein beads) efficiently induced the proliferation of epidermal cells.

These results on FGF2, FGF7 and EGF protein beads have been published (Ijiri et al. (2009) Biomaterials 30, 4297-4308).

      US patent number 7,432,347 (Oct. 7, 2008)
      US patent number 7,619,060 (Nov. 17, 2009)

    Recent papers:
Nature 446, 97-101 (2007)
2. Journal of Biological Chemistry 282, 17289-17296 (2007)

     3. Biomaterials 30, 4297-4308 (2009)       
     4. Tissue Engineering Part A, 16, 3329-3341 (2010)

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Please contact to Hajime Mori, Ph.D.


Professor (Kyoto Institute of Technology)